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Quantitative real-time PCR (QRT-PCR) was performed using the One Step SYBR Ex Taq qRT-PCR Kit (TaKaRa Bio inc, Japan) on a Rotor-Gene 6000 instrument (Corbett Research, Australia) under the following cycling conditions: initial denaturation at 95 C for 30 sec, followed by 40 cycles of 95 C for 30 sec and 60 C for 30 sec

However, several new drugs, small molecules, and monoclonal antibodies have been identified that directly target oncogenic factors

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24 h after hypoxia episode animals were decapitated without anesthesia for the measurements of oxidative stress biomarkers